{"id":10005,"date":"2016-09-30T08:02:10","date_gmt":"2016-09-30T08:02:10","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=10005"},"modified":"2016-09-30T08:02:10","modified_gmt":"2016-09-30T08:02:10","slug":"mitochondrial-proteins-as-possible-biomarkers-for-subgroups-of-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/mitochondrial-proteins-as-possible-biomarkers-for-subgroups-of-cfs\/","title":{"rendered":"Mitochondrial proteins as possible biomarkers for subgroups of CFS"},"content":{"rendered":"

Research abstract:<\/strong><\/h3>\n

Chronic fatigue syndrome (CFS) is a debilitating and complex disorder\u00a0characterized by unexplained fatigue not improved by rest. An area of\u00a0investigation is the likely connection of CFS with defective\u00a0mitochondrial function.<\/p>\n

In a previous work, we investigated the\u00a0proteomic salivary profile <\/a>in a couple of monozygotic twins discordant\u00a0for CFS. Following this work, we analyzed mitochondrial proteins<\/a> in the\u00a0same couple of twins. Nano-liquid chromatography electrospray ionization
\nmass spectrometry (nano-LC-MS) was used to study the mitochondria\u00a0extracted from platelets of the twins.<\/p>\n

Subsequently, we selected three\u00a0proteins that were validated using western blot analysis in a big cohort\u00a0of subjects (n=45 CFS; n=45 healthy), using whole saliva (WS). The\u00a0selected proteins were as follows: aconitate hydratase (ACON), ATP\u00a0synthase subunit beta (ATPB) and malate dehydrogenase (MDHM). Results\u00a0for ATPB and ACON confirmed their upregulation in CFS. However, the MDHM\u00a0alteration was not confirmed.<\/p>\n

Thereafter, seeing the great variability\u00a0of clinical features of CFS patients, we decided to analyze the\u00a0expression of our proteins after splitting patients according to\u00a0clinical parameters. For each marker, the values were actually higher in\u00a0the group of patients who had clinical features similar to the ill twin.<\/p>\n

In conclusion, these results suggest that our potential markers could be\u00a0one of the criteria to be taken into account for helping in diagnosis.\u00a0 Furthermore, the identification of biomarkers present in particular\u00a0subgroups of CFS patients may help in shedding light upon the complex\u00a0entity of CFS. Moreover, it could help in developing tailored
\ntreatments.<\/p>\n

Bottom-up proteomics suggests an association between differential\u00a0expression of mitochondrial proteins and chronic fatigue syndrome<\/a>, by\u00a0F Ciregia, L Kollipara, L Giusti, RP Zahedi, C\u00a0Giacomelli, MR Mazzoni, G Giannaccini, P Scarpellini,\u00a0A Urbani, A Sickmann, A Lucacchini, L Bazzichi in<\/span> Translational Psychiatry<\/em> Translational Vol. 6, #9, p e904<\/p>\n

Supplementary Information<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Research abstract: Chronic fatigue syndrome (CFS) is a debilitating and complex disorder\u00a0characterized by unexplained fatigue not improved by rest. An area of\u00a0investigation is the likely connection of CFS with defective\u00a0mitochondrial function. In a previous work, we investigated the\u00a0proteomic salivary profile … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[726,18,3156],"class_list":["post-10005","post","type-post","status-publish","format-standard","hentry","category-news","tag-biomarkers","tag-gene-expression","tag-mitochondrial-proteins"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-2Bn","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/10005","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=10005"}],"version-history":[{"count":3,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/10005\/revisions"}],"predecessor-version":[{"id":10033,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/10005\/revisions\/10033"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=10005"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=10005"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=10005"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}