{"id":11387,"date":"2017-01-13T11:20:59","date_gmt":"2017-01-13T11:20:59","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=11387"},"modified":"2017-01-13T11:20:59","modified_gmt":"2017-01-13T11:20:59","slug":"%ef%bb%bfthe-association-of-fecal-microbiota-fecal-blood-serum-urine-metabolites-in-mecfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/%ef%bb%bfthe-association-of-fecal-microbiota-fecal-blood-serum-urine-metabolites-in-mecfs\/","title":{"rendered":"\ufeffThe association of fecal microbiota & fecal, blood serum & urine metabolites in ME\/CFS"},"content":{"rendered":"

Research abstract:<\/strong><\/h3>\n

INTRODUCTION:<\/p>\n

The human gut microbiota has the ability to modulate host metabolism<\/a>. Metabolic profiling of the microbiota and the host biofluids may determine associations significant of a host\u2013microbe relationship. Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a long-term disorder of fatigue that is poorly understood, but has been linked to gut problems and altered microbiota<\/a>.<\/p>\n

OBJECTIVES<\/p>\n

Find changes in fecal microbiota and metabolites in ME\/CFS and determine their association with blood serum and urine metabolites.<\/p>\n

METHODS<\/p>\n

A workflow was developed that correlates microbial counts with fecal, blood serum and urine metabolites quantitated by high-throughput 1H NMR spectroscopy. The study consists of thirty-four females with ME\/CFS (34.9\u2009\u00b1\u20091.8 SE years old) and twenty-five non-ME\/CFS female (33.0\u2009\u00b1\u20091.6 SE years old).<\/p>\n

RESULTS<\/p>\n

The workflow was validated using the non-ME\/CFS cohort where fecal short chain fatty acids (SCFA) were associated with serum and urine metabolites indicative of host metabolism changes enacted by SCFA.<\/p>\n

In the ME\/CFS cohort a decrease in fecal lactate and an increase in fecal butyrate, isovalerate and valerate were observed along with an increase in Clostridium spp. and a decrease in Bacteroides spp. These differences were consistent with an increase in microbial fermentation of fiber and amino acids to produce SCFA in the gut of ME\/CFS patients. Decreased fecal amino acids positively correlated with substrates of gluconeogenesis and purine synthesis in the serum of ME\/CFS patients.<\/p>\n

CONCLUSION<\/p>\n

Increased production of SCFA by microbial fermentation in the gut of ME\/CFS patients may be associated with deleterious effects on the host energy metabolism.<\/p>\n

The association of fecal microbiota and fecal, blood serum and urine metabolites in myalgic encephalomyelitis\/chronic fatigue syndrome<\/a>, by Christopher W. Armstrong, Neil R. McGregor, Donald, P. Lewis, Henry L. Butt, Paul R Gooley in<\/span> Metabolomics<\/em>, January 2017 13:8 [Published online 12 Dec 2016]<\/p>\n","protected":false},"excerpt":{"rendered":"

Research abstract: INTRODUCTION: The human gut microbiota has the ability to modulate host metabolism. Metabolic profiling of the microbiota and the host biofluids may determine associations significant of a host\u2013microbe relationship. Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a long-term disorder … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[3413,3297,3084,41,3433,3353],"class_list":["post-11387","post","type-post","status-publish","format-standard","hentry","category-news","tag-amino-acids","tag-chris-armstrong","tag-dr-neil-mcgregor","tag-energy-metabolism","tag-fecal-microbiota","tag-short-chain-fatty-acids"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-2XF","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11387","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=11387"}],"version-history":[{"count":2,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11387\/revisions"}],"predecessor-version":[{"id":11389,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11387\/revisions\/11389"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=11387"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=11387"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=11387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}