{"id":11668,"date":"2017-01-31T09:11:52","date_gmt":"2017-01-31T09:11:52","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=11668"},"modified":"2017-03-31T09:28:05","modified_gmt":"2017-03-31T09:28:05","slug":"naviaux-gordon-respond-to-criticisms-of-their-metabolomics-research","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/naviaux-gordon-respond-to-criticisms-of-their-metabolomics-research\/","title":{"rendered":"Naviaux &#038; Gordon respond to criticisms of their metabolomics research"},"content":{"rendered":"<p><a href=\"http:\/\/www.pnas.org\/content\/114\/6\/E911.extract\" target=\"_blank\">Reply to Roerink et al.: Metabolomics of chronic fatigue syndrome<\/a>, by\u00a0Robert K. Naviaux and Eric Gordon\u00a0<span style=\"text-decoration: underline;\">in<\/span> <em>PNAS<\/em> 2017 114 (6) E911-E912; [published ahead of print January 26, 2017]<\/p>\n<p>We thank Roerink et al. (1) <a href=\"http:\/\/www.pnas.org\/content\/114\/6\/E910.extract\" target=\"_blank\">for their comments.<\/a> We respond to their two\u00a0points in order.<\/p>\n<p>Their first point asked about the effect of physical\u00a0 activity on sphingolipids.\u00a0 The\u00a0phingolipid response to exercise is\u00a0 complex. It differs in healthy trained and untrained individuals and has\u00a0not yet been studied using methods that can distinguish the classes of\u00a0sphingosines, ceramides, sphingomyelins, and glycosphingolipids measured<br \/>\nin our analysis of myalgic encephalomyelitis (ME)\/chronic fatigue\u00a0\u00a0syndrome (CFS) (2)&#8230;<\/p>\n<p>Second, Roerink et al. (1) raise questions about the standard\u00a0statistical methods used in metabolomics studies. We used multivariate analysis by partial least squares discriminate analysis (PLSDA), area\u00a0under the receiver operator characteristic (AUROC) curve analysis,<br \/>\nrandom forest methods for biomarker discovery, repeated double\u00a0cross-validation, and permutation analysis&#8230;<\/p>\n<p><a href=\"http:\/\/forums.phoenixrising.me\/index.php?threads\/metabolome-of-chronic-fatigue-syndrome-jos-w-m-van-der.48979\/\" target=\"_blank\">Read the full response<\/a> at bottom of page<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Reply to Roerink et al.: Metabolomics of chronic fatigue syndrome, by\u00a0Robert K. Naviaux and Eric Gordon\u00a0in PNAS 2017 114 (6) E911-E912; [published ahead of print January 26, 2017] We thank Roerink et al. (1) for their comments. We respond to &hellip; <a href=\"https:\/\/wames.org.uk\/cms-english\/naviaux-gordon-respond-to-criticisms-of-their-metabolomics-research\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":true,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[2714,2559,1262],"class_list":["post-11668","post","type-post","status-publish","format-standard","hentry","category-news","tag-dr-eric-gordon","tag-dr-robert-naviaux","tag-metabolomics"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-32c","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11668","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=11668"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11668\/revisions"}],"predecessor-version":[{"id":12460,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/11668\/revisions\/12460"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=11668"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=11668"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=11668"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}