{"id":15053,"date":"2018-03-08T08:21:59","date_gmt":"2018-03-08T08:21:59","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=15053"},"modified":"2018-03-08T08:25:49","modified_gmt":"2018-03-08T08:25:49","slug":"investigation-of-mast-cell-toll-like-receptor-3-in-cfs-me","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/investigation-of-mast-cell-toll-like-receptor-3-in-cfs-me\/","title":{"rendered":"Investigation of mast cell toll-like receptor 3 in CFS\/ME"},"content":{"rendered":"

Research abstract:<\/strong><\/h3>\n

Investigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry<\/a>, by C Balinas, T Nguyen, S\u00a0Johnston, P\u00a0Smith, D\u00a0Staines, S\u00a0Marshall-Gradisnik in<\/span> Asian Pac J Allergy Immunol<\/em>. 2017 Dec 10 [Epub ahead of print]<\/p>\n

BACKGROUND:<\/p>\n

\"\"Viral infections and hypersensitivities are commonly reported by Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis (CFS\/ME) patients. Mast Cells<\/a> (MC) uniquely mediate type 1 hypersensitivities and resolve viral infections via toll-like receptor<\/a> 3 (TLR3).<\/p>\n

OBJECTIVE:<\/p>\n

To characterise and compare mast cell progenitors (MCPs) in CFS\/ME participants with a known MC disorder, Systemic mastocytosis (SM), and secondly, to investigate the role of MC TLR3 in CFS\/ME participants following Polyinosinic:polycytidylic acid (Poly I:C) stimulation.<\/p>\n

METHODS:<\/p>\n

A total of 11 International Consensus Criteria defined CFS\/ME participants (40.42 \u00b1 10.31), 9 World Health Organisation defined systemic mastocytosis (SM) participants (47.00 \u00b1 10.37) and 12 healthy controls (HC) (36.36 \u00b1 9.88) were included. Following autoMACS magnetic separation, CD117+\/Lin-MCPs were stimulated with Poly I:C for 24hr. MCP purity (CD117 and Lin2), maturity (CD34 and Fc\u03b5RI), interaction receptors and ligands (CD154 and HLA-DR), and SM-specific (CD2 and CD25) markers were measured using flow cytometry.<\/p>\n

RESULTS:<\/p>\n

There was a significant decrease in HLA-DR+\/CD154- expression between CFS\/ME and SM groups pre and post Poly I:C stimulation. There were no significant differences in maturity MCPs, CD154, and CD2\/CD25 expression between groups pre and post Poly I:C stimulation.<\/p>\n

CONCLUSION:<\/p>\n

This pilot investigation provides a novel methodology to characterise MCPs in a rapid, inexpensive and less invasive fashion.<\/p>\n

We report a significant decrease in HLA-DR+\/CD154- expression between CFS\/ME and SM participants, and an observed increase in HLA-DR-\/CD154+ expression post Poly I:C stimulation in CFS\/ME participants.<\/p>\n

Peripheral MCPs may be present in CFS\/ME pathophysiology, however further investigation is required to determine their immunological role.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Research abstract: Investigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry, by C Balinas, T Nguyen, S\u00a0Johnston, P\u00a0Smith, D\u00a0Staines, S\u00a0Marshall-Gradisnik in Asian … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[4125,2761,1249,661,4124,4123,2785,2676,2783,4126,1610,3601],"class_list":["post-15053","post","type-post","status-publish","format-standard","hentry","category-news","tag-c-balinas","tag-dr-samantha-johnston","tag-dr-sonya-marshall-gradisnik","tag-immune-dysfunction","tag-mast-cells","tag-mcps","tag-p-smith","tag-prof-donald-staines","tag-t-nguyen","tag-tlr3","tag-toll-like-receptors","tag-white-blood-cells"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-3UN","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/15053","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=15053"}],"version-history":[{"count":3,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/15053\/revisions"}],"predecessor-version":[{"id":15794,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/15053\/revisions\/15794"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=15053"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=15053"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=15053"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}