{"id":16295,"date":"2018-04-30T06:33:22","date_gmt":"2018-04-30T06:33:22","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=16295"},"modified":"2018-04-30T06:35:44","modified_gmt":"2018-04-30T06:35:44","slug":"integration-of-dna-methylation-health-scores-identifies-subtypes-in-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/integration-of-dna-methylation-health-scores-identifies-subtypes-in-me-cfs\/","title":{"rendered":"Integration of DNA methylation &#038; health scores identifies subtypes in ME\/CFS"},"content":{"rendered":"<h3><strong>Research abstract:<\/strong><\/h3>\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29692205\" target=\"_blank\" rel=\"noopener\">Integration of DNA methylation &amp; health scores identifies subtypes in myalgic encephalomyelitis\/chronic fatigue syndrome<\/a>, by Wilfred C de Vega, Lauren Erdman, Suzanne D Vernon, Anna Goldenberg &amp; Patrick O McGowan in Epigenomics. 2018 Apr 25. doi: 10.2217\/epi-2017-0150. [Epub ahead of print]<\/p>\n<p style=\"padding-left: 30px;\"><em>If your body cannot methylate properly, toxins build up in your bloodstream and will eventually cause disease. &#8230; In fact, methylation can turn genes on or off, which can be good or bad for our health, depending on the gene.\u00a0<\/em> <a href=\"http:\/\/www.revolutionhealth.org\/what-is-methylation-and-why-should-you-care\/\" target=\"_blank\" rel=\"noopener\">Read<\/a><a href=\"http:\/\/www.revolutionhealth.org\/what-is-methylation-and-why-should-you-care\/\" target=\"_blank\" rel=\"noopener\"> mo<\/a><a href=\"http:\/\/www.revolutionhealth.org\/what-is-methylation-and-why-should-you-care\/\" target=\"_blank\" rel=\"noopener\">r<\/a><a href=\"http:\/\/www.revolutionhealth.org\/what-is-methylation-and-why-should-you-care\/\" target=\"_blank\" rel=\"noopener\">e<\/a><\/p>\n<p>AIM:<\/p>\n<p>To identify subtypes in myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) based on <a href=\"https:\/\/en.wikipedia.org\/wiki\/DNA_methylation\" target=\"_blank\" rel=\"noopener\">DNA methylation<\/a> profiles and health scores.<\/p>\n<p>METHODS:<\/p>\n<p>DNA methylome profiles in immune cells were integrated with symptomatology from 70 women with ME\/CFS using similarity network fusion to identify subtypes.<\/p>\n<p>RESULTS:<\/p>\n<p>We discovered four ME\/CFS subtypes associated with DNA methylation modifications in 1939 CpG sites, three <a href=\"https:\/\/www.rand.org\/health\/surveys_tools\/mos\/36-item-short-form.html\" target=\"_blank\" rel=\"noopener\">RAND-36<\/a> categories and five <a href=\"http:\/\/me-pedia.org\/wiki\/DePaul_Symptom_Questionnaire\" target=\"_blank\" rel=\"noopener\">DePaul Symptom Questionnaire<\/a> measures. Methylation patterns of immune response genes and differences in physical functioning and postexertional malaise differentiated the subtypes.<\/p>\n<p>CONCLUSION:<\/p>\n<p>ME\/CFS subtypes are associated with specific DNA methylation differences and health symptomatology and provide additional evidence of the potential relevance of metabolic and immune differences in ME\/CFS with respect to specific symptoms.<\/p>\n<h3><strong>Summary points<\/strong><\/h3>\n<ul>\n<li>DNA methylation data and health score data using the RAND-36 survey and the DePaul Symptom Questionnaire were collected in 70 women diagnosed with myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS).<\/li>\n<li>Potential clinical subtypes integrating DNA methylation differences in ME\/CFS biomarkers (4699 sites in total) and health score data were identified using similarity network fusion, a clustering approach that preserves the data structures unique to each level of data.<\/li>\n<li>Similarity network fusion analysis revealed the presence of four clinical subtypes among the ME\/CFS patients based on methylation differences in 1939 sites, three RAND-36 categories and five DePaul measures (normalized mutual information [NMI] &gt;0.2, Kruskal\u2013Wallis FDR &lt;0.05).<\/li>\n<li>RAND-36 scores that significantly contributed to clustering corresponded to physical functioning (NMI = 0.26), social functioning (NMI = 0.23) and role limitations due to physical health (NMI = 0.23).<\/li>\n<li>DePaul Symptom Questionnaire measures that were significantly different between ME\/CFS clinical subtypes were associated with frequency of postexertional malaise, severity of pain\/muscle weakness and severity of unrefreshing sleep.<\/li>\n<li>Gene set enrichment analysis of the top 50 sites that contributed to differentiating the ME\/CFS subtypes were mainly annotated to immune response.<\/li>\n<li>The results are consistent with previous work that described immune and metabolic dysfunction in ME\/CFS.<\/li>\n<li>The described subtypes of our study are also generally consistent with previous ME\/CFS work that identified immune gene and degree of symptom severity as highly relevant factors in differentiating ME\/CFS subtypes.<\/li>\n<\/ul>\n<p><a href=\"http:\/\/sci-hub.hk\/10.2217\/epi-2017-0150\" target=\"_blank\" rel=\"noopener\">Read full paper<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Research abstract: Integration of DNA methylation &amp; health scores identifies subtypes in myalgic encephalomyelitis\/chronic fatigue syndrome, by Wilfred C de Vega, Lauren Erdman, Suzanne D Vernon, Anna Goldenberg &amp; Patrick O McGowan in Epigenomics. 2018 Apr 25. doi: 10.2217\/epi-2017-0150. [Epub &hellip; <a href=\"https:\/\/wames.org.uk\/cms-english\/integration-of-dna-methylation-health-scores-identifies-subtypes-in-me-cfs\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[4382,4381,603,2007,1009,35,1112,4383,4380,3210,1111],"class_list":["post-16295","post","type-post","status-publish","format-standard","hentry","category-news","tag-anna-goldenberg","tag-clinical-subtyping","tag-depaul-symptom-questionnaire","tag-dna-methylation","tag-dr-patrick-o-mcgowan","tag-dr-suzanne-vernon","tag-epigenetics","tag-lauren-erdman","tag-rand-36-short-form-survey","tag-sub-groups","tag-wilfred-c-de-vega"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-4eP","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16295","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=16295"}],"version-history":[{"count":3,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16295\/revisions"}],"predecessor-version":[{"id":16328,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16295\/revisions\/16328"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=16295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=16295"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=16295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}