{"id":16888,"date":"2018-06-01T20:28:42","date_gmt":"2018-06-01T20:28:42","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=16888"},"modified":"2020-02-11T20:30:22","modified_gmt":"2020-02-11T20:30:22","slug":"decreased-expression-of-trpm3-machrm3-in-the-small-intestine-in-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/decreased-expression-of-trpm3-machrm3-in-the-small-intestine-in-me-cfs\/","title":{"rendered":"Decreased Expression of TRPM3 & mAChRM3 in the small intestine in ME\/CFS"},"content":{"rendered":"

Decreased Expression of TRPM3 and mAChRM3 in the Small Intestine in Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis<\/a>, by\u00a0Sonya Marshall-Gradisnik, Marshall Fretel, Natalie Eaton, Helene Cabanas, Cassandra Balinas, Vinod Gopalan, Daniel Petersen, Rachel Passmore, Kevin Tang, Mazhar Haque, Alfred Lam, Donald Staines in<\/span>\u00a0IJCM<\/em> Vol.9 No.5, May 2018,\u00a0PP. 467-480<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Introduction:<\/strong>\u00a0Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis (CFS\/ME) is often associated with gastrointestinal disturbance and inflammatory markers; however, there have been no histological studies performed in the small intestine from CFS\/ME patients.<\/p>\n

The aim of this investigation was to assess the expression of certain inflammatory markers and inflammatory receptors, namely transient receptor potential melastin 3 (TRPM3) ion channels and muscarinic acetylcholine M3 (mAChRM3) receptors, in small intestinal tissues in a case controlled study comprising a CFS\/ME patient and a healthy non-fatigued control.<\/p>\n

Method:<\/strong>\u00a0Immunohistochemistry was performed on a small intestinal biopsy from a CFS\/ME patient (age = 50; female) with self-reported symptoms of gastrointestinal disturbance and a non-fatigued control (NFC), (age = 28; female). Semi-quantitative analysis of expression was undertaken for interferon-gamma (IFNy), interleukin-1 alpha (IL-1\u03b1), tumour necrosis factor-alpha (TNF\u03b1), TRPM3 ion channels and mAChRM3 acetylcholine receptors.<\/p>\n

Results:<\/strong>\u00a0There was significantly decreased expression of TRPM3 in the CFS\/ME patient (35%\u00a0\u00b1\u00a09%) and a significant decrease in mAChRM3 in the CFS\/ME patient (54%\u00a0\u00b1\u00a09%). There was no difference in IL-1\u03b1 between CFS\/ME patient and NFC, however; there was an increase in IFNy (13%\u00a0\u00b1\u00a06%) in the CFS\/ME patient compared to NFC. There was a difference observed in TNF\u03b1 in CFS\/ME compared to NFC.<\/p>\n

Conclusion:<\/strong> Differences were noted in the expression of specific TRP ion channels and cholinergic receptors in CFS\/ME compared with NFC, with CFS\/ME demonstrating decreased TRPM3 and mAChRM3. Further, IFNy was increased, and TNF\u03b1\u00a0decreased, in the small intestine of the CFS\/ME patient with reported gastrointestinal disturbance.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Decreased Expression of TRPM3 and mAChRM3 in the Small Intestine in Chronic Fatigue Syndrome\/Myalgic Encephalomyelitis, by\u00a0Sonya Marshall-Gradisnik, Marshall Fretel, Natalie Eaton, Helene Cabanas, Cassandra Balinas, Vinod Gopalan, Daniel Petersen, Rachel Passmore, Kevin Tang, Mazhar Haque, Alfred Lam, Donald Staines in\u00a0IJCM … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-16888","post","type-post","status-publish","format-standard","hentry","category-news"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-4oo","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16888","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=16888"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16888\/revisions"}],"predecessor-version":[{"id":24389,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/16888\/revisions\/24389"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=16888"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=16888"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=16888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}