{"id":17541,"date":"2018-07-30T10:36:38","date_gmt":"2018-07-30T10:36:38","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=17541"},"modified":"2018-07-30T10:36:38","modified_gmt":"2018-07-30T10:36:38","slug":"identification-of-me-cfs-associated-dna-methylation-patterns","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/identification-of-me-cfs-associated-dna-methylation-patterns\/","title":{"rendered":"Identification of ME\/CFS-associated DNA methylation patterns"},"content":{"rendered":"

Identification of Myalgic Encephalomyelitis\/Chronic Fatigue\u00a0Syndrome-associated DNA methylation patterns<\/a>, by\u00a0Malav S. Trivedi, Elisa Oltra, Leonor Sarria, Natasha\u00a0Rose, Vladimir Beljanski, Mary Ann Fletcher, Nancy\u00a0\u00a0G. Klimas, Lubov Nathanson in<\/span>\u00a0PLoS One<\/em>\u00a0Vol 13, #7, p e0201066 [Published: July 23, 2018]<\/p>\n

Abstract:<\/strong><\/p>\n

Background:<\/strong>
\nMyalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS) is a complex
\ncondition involving multiple organ systems and characterized by\u00a0persistent\/ relapsing debilitating fatigue, immune dysfunction,\u00a0neurological problems, and other symptoms not curable for at least 6\u00a0months.<\/p>\n

\"\"Disruption of DNA methylation patterns<\/a> has been tied to various\u00a0immune and neurological diseases; however, its status in ME\/CFS remains\u00a0uncertain. Our study aimed at identifying changes in the DNA methylation\u00a0patterns that associate with ME\/CFS.<\/p>\n

Methods:<\/strong>
\nWe extracted genomic DNA from peripheral blood mononuclear cells from 13\u00a0ME\/CFS study subjects and 12 healthy controls and measured global DNA\u00a0methylation by ELISA-like method and site-specific methylation status\u00a0using Illumina MethylationEPIC microarrays. Pyrosequencing validation\u00a0included 33 ME\/CFS cases and 31 controls from two geographically distant
\ncohorts.<\/p>\n

Results:<\/strong>
\nGlobal DNA methylation levels of ME\/CFS cases were similar to those of\u00a0controls. However, microarray-based approach allowed detection of 17,296\u00a0differentially methylated CpG sites in 6,368 genes across regulatory\u00a0elements and within coding regions of genes. Analysis of DNA methylation\u00a0in promoter regions revealed 307 differentially methylated promoters.\u00a0Ingenuity pathway analysis indicated that genes associated with
\ndifferentially methylated promoters participated in at least 15\u00a0different pathways mostly related to cell signaling with a strong immune\u00a0component.<\/p>\n

Conclusions:<\/strong>
\nThis is the first study that has explored genome-wide epigenetic <\/a>changes\u00a0associated with ME\/CFS using the advanced Illumina MethylationEPIC\u00a0microarrays covering about 850,000 CpG sites in two geographically\u00a0distant cohorts of ME\/CFS cases and matched controls. Our results are\u00a0aligned with previous studies that indicate a dysregulation of the
\nimmune system in ME\/CFS. They also suggest a potential role of\u00a0epigenetic de-regulation in the pathobiology of ME\/CFS.<\/p>\n

We propose\u00a0screening of larger cohorts of ME\/CFS cases to determine the external
\nvalidity of these epigenetic changes in order to implement them as\u00a0possible diagnostic markers in clinical setting.<\/p>\n","protected":false},"excerpt":{"rendered":"

Identification of Myalgic Encephalomyelitis\/Chronic Fatigue\u00a0Syndrome-associated DNA methylation patterns, by\u00a0Malav S. Trivedi, Elisa Oltra, Leonor Sarria, Natasha\u00a0Rose, Vladimir Beljanski, Mary Ann Fletcher, Nancy\u00a0\u00a0G. Klimas, Lubov Nathanson in\u00a0PLoS One\u00a0Vol 13, #7, p e0201066 [Published: July 23, 2018] Abstract: Background: Myalgic Encephalomyelitis\/Chronic Fatigue … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1878,4541,1112,4543,4546,4542,3631,4544,3377,4545],"class_list":["post-17541","post","type-post","status-publish","format-standard","hentry","category-news","tag-dr-nancy-klimas","tag-epigenetic-deregulation","tag-epigenetics","tag-leonor-sarria","tag-lubov-nathanson","tag-malav-s-trivedi","tag-mary-ann-fletcher","tag-natasha-rose","tag-prof-elisa-oltra","tag-vladimir-beljanski"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-4yV","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/17541","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=17541"}],"version-history":[{"count":2,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/17541\/revisions"}],"predecessor-version":[{"id":17563,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/17541\/revisions\/17563"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=17541"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=17541"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=17541"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}