{"id":19304,"date":"2019-02-01T11:11:41","date_gmt":"2019-02-01T11:11:41","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=19304"},"modified":"2019-02-01T11:11:42","modified_gmt":"2019-02-01T11:11:42","slug":"changes-in-the-transcriptome-of-circulating-immune-cells-of-a-n-z-cohort-with-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/changes-in-the-transcriptome-of-circulating-immune-cells-of-a-n-z-cohort-with-me-cfs\/","title":{"rendered":"Changes in the transcriptome of circulating immune cells of a N Z cohort with ME\/CFS"},"content":{"rendered":"

Changes in the transcriptome of circulating immune cells of a New Zealand cohort with myalgic encephalomyelitis\/chronic fatigue syndrome<\/a>, by Eiren Sweetman, Margaret Ryan, Christina Edgar, Angus MacKay, Rosamund Vallings,\u00a0Warren Tate in International Journal of Immunopathology and Pharmacology vol 33 2019\u00a0[Published January 11, 2019]<\/h3>\n

 <\/p>\n

Research abstract:<\/strong>
\nMyalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a poorly understood disease affecting 0.2%\u20132% of the global population. To gain insight into the pathophysiology of ME\/CFS in New Zealand, we examined the transcriptomes<\/a> of peripheral blood mononuclear cells by RNA<\/a>-seq analysis in a small well-characterized patient group (10 patients), with age\/gender-matched healthy controls (10 control subjects).<\/p>\n

\"\"<\/a><\/p>\n

Twenty-seven gene transcripts<\/a> were increased 1.5- to sixfold and six decreased three- to sixfold in the patient group (P\u2009<\u20090.01). The top enhanced gene transcripts, IL8, NF\u039aBIA and TNFAIP3, are functionally related to inflammation, and significant changes were validated for IL8 and NF\u039aBIA by quantitative polymerase chain reaction<\/a> (qPCR).<\/p>\n

Functional network analysis of the altered gene transcripts (P\u2009<\u20090.01) detected interactions between the products related to inflammation, circadian clock function<\/a>, metabolic dysregulation<\/a>, cellular stress responses<\/a> and mitochondrial function<\/a>. Ingenuity pathway analysis (P\u2009<\u20090.05) provided further insights into the dysfunctional physiology, highlighting stress and inflammation pathways.<\/p>\n

This analysis provides novel insights into the molecular changes in ME\/CFS and contributes to the understanding of the pathophysiological mechanisms of the disease.<\/p>\n

From the conclusion:<\/strong><\/p>\n

Our exploratory approach has enabled us to obtain a rich differentially expressed gene dataset to identify changed biology in ME\/CFS. We have identified the circadian rhythm dysregulation pathway as a new possible underlying cause of the unrefreshing sleep, fatigue and metabolic abnormalities seen in ME\/CFS. Furthermore, impaired mitochondrial function and resulting oxidative stress, coupled with chronic immune-inflammatory signalling, provides a compelling explanation for the fatigue, cognitive dysfunction and post-exertion malaise experienced in ME\/CFS.<\/p>\n

Therefore, this study is a further step towards gaining an understanding of the disease process and identifying putative biomarkers to support clinical diagnosis. The biological pathways identified offer a rational explanation of the complex and often multi-systemic nature of ME\/CFS.<\/p>\n","protected":false},"excerpt":{"rendered":"

Changes in the transcriptome of circulating immune cells of a New Zealand cohort with myalgic encephalomyelitis\/chronic fatigue syndrome, by Eiren Sweetman, Margaret Ryan, Christina Edgar, Angus MacKay, Rosamund Vallings,\u00a0Warren Tate in International Journal of Immunopathology and Pharmacology vol 33 2019\u00a0[Published … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[4844,4849,4845,1320,4851,4848,447,4850,4846,168,306,4117,4847],"class_list":["post-19304","post","type-post","status-publish","format-standard","hentry","category-news","tag-angus-mackay","tag-christina-edgar","tag-circadian-rhythm","tag-dr-rosamund-vallings","tag-eiren-sweetman","tag-gene-transcripts","tag-inflammation","tag-margaret-ryan","tag-metabolic-dysregulation","tag-mitochondrial-dysfunction","tag-oxidative-stress","tag-prof-warren-tate","tag-transcriptome"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-51m","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/19304","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=19304"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/19304\/revisions"}],"predecessor-version":[{"id":19492,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/19304\/revisions\/19492"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=19304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=19304"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=19304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}