{"id":20228,"date":"2019-03-27T07:45:21","date_gmt":"2019-03-27T07:45:21","guid":{"rendered":"http:\/\/wames.org.uk\/cms-english\/?p=20228"},"modified":"2019-03-27T07:45:21","modified_gmt":"2019-03-27T07:45:21","slug":"whole-blood-human-transcriptome-and-virome-analysis-of-me-cfs-patients-experiencing-post-exertional-malaise-following-cardiopulmonary-exercise-testing","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/whole-blood-human-transcriptome-and-virome-analysis-of-me-cfs-patients-experiencing-post-exertional-malaise-following-cardiopulmonary-exercise-testing\/","title":{"rendered":"Whole blood human transcriptome and virome analysis of ME\/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing"},"content":{"rendered":"

Whole blood human transcriptome and virome analysis of ME\/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing<\/a>, by Jerome Bouquet, Tony Li, Jennifer L Gardy, Xiaoying Kang, Staci Stevens, Jared Stevens, Mark VanNess, Christopher Snell, James Potts, Ruth R Miller, Muhammad Morshed, Mark McCabe, Shoshana Parker, Miguel Uyaguari,\u00a0 Patrick Tang, Theodore Steiner, Wee-Shian Chan, Astrid-Marie De Souza, Andre Mattman, David M Patrick , Charles Y Chiu in<\/span> PLOS One<\/em> March 21, 2019<\/h3>\n

Research abstract:<\/strong>
\nMyalgic encephalomyelitis \/ chronic fatigue syndrome (ME\/CFS) is a syndrome of unknown etiology characterized by profound fatigue exacerbated by physical activity, also known as post-exertional malaise (PEM).<\/p>\n

Previously, we did not detect evidence of immune dysregulation or virus reactivation outside of PEM periods. Here we sought to determine whether cardiopulmonary exercise stress testing of ME\/CFS patients could trigger such changes.<\/p>\n

\"\"<\/a>ME\/CFS patients (n = 14) and matched sedentary controls (n = 11) were subjected to cardiopulmonary exercise on 2 consecutive days and followed up to 7 days post-exercise, and longitudinal whole blood samples analyzed by RNA-seq.<\/p>\n

Although ME\/CFS patients showed significant worsening of symptoms following exercise versus controls, with 8 of 14 ME\/CFS patients showing reduced oxygen consumption ( ) on day 2, transcriptome analysis yielded only 6 differentially expressed gene (DEG) candidates when comparing ME\/CFS patients to controls across all time points. None of the DEGs were related to immune signaling, and no DEGs were found in ME\/CFS patients before and after exercise. Virome composition (P = 0.746 by chi-square test) and number of viral reads (P = 0.098 by paired t-test) were not significantly associated with PEM.<\/p>\n

These observations do not support transcriptionally-mediated immune cell dysregulation or viral reactivation in ME\/CFS patients during symptomatic PEM episodes.<\/p>\n","protected":false},"excerpt":{"rendered":"

Whole blood human transcriptome and virome analysis of ME\/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing, by Jerome Bouquet, Tony Li, Jennifer L Gardy, Xiaoying Kang, Staci Stevens, Jared Stevens, Mark VanNess, Christopher Snell, James Potts, Ruth R Miller, … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[3049,4931,4930,516,4933,4539,4932,4934,4924,4604,4660,4926,4927,4925,4658,584,585,654,4657,4656,2074,4928,4922,4935,4936,4929,4923],"class_list":["post-20228","post","type-post","status-publish","format-standard","hentry","category-news","tag-2-day-cpet","tag-andre-mattman","tag-astrid-marie-se-souza","tag-cardiopulmonary-exercise-testing","tag-charles-y-chiu","tag-christopher-r-snell","tag-david-m-patrick","tag-immune-cell-dysregulation","tag-james-potts","tag-jared-stevens","tag-jennifer-l-gardy","tag-mark-mccabe","tag-miguel-uyaguari","tag-muhammed-morshed","tag-patrick-tang","tag-pem","tag-post-exertional-malaise","tag-prof-mark-vanness","tag-ruth-r-miller","tag-shoshana-m-parker","tag-staci-r-stevens","tag-theodore-steiner","tag-tony-li","tag-viral-reactivation","tag-virome-analysis","tag-wee-shian-chan","tag-xiaoying-kang"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-5gg","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/20228","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=20228"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/20228\/revisions"}],"predecessor-version":[{"id":20272,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/20228\/revisions\/20272"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=20228"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=20228"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=20228"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}