{"id":21767,"date":"2019-07-15T07:15:07","date_gmt":"2019-07-15T07:15:07","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=21767"},"modified":"2019-07-15T07:15:07","modified_gmt":"2019-07-15T07:15:07","slug":"micrornas-as-biomarkers-of-pain-intensity-in-patients-with-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/micrornas-as-biomarkers-of-pain-intensity-in-patients-with-cfs\/","title":{"rendered":"MicroRNAs as biomarkers of pain intensity in patients with CFS"},"content":{"rendered":"

MicroRNAs as biomarkers of pain intensity in patients with chronic fatigue syndrome, <\/a>by Hadeel A Al\u2010Rawaf, Ahmad H Alghadir, Sami A Gabr in<\/span> Pain Pract<\/em>. 2019 Jul 8. doi: 10.1111\/papr.12817. [Epub ahead of print]<\/h3>\n

Research abstract:<\/strong><\/h3>\n

\"\"<\/a>BACKGROUND:<\/p>\n

Numerous experimental models have shown that microRNAs<\/a> play an important role in regulating pain-processing in clinical pain disorders. In this study, we evaluated a set of micro-RNAs as diagnostic biomarkers of pain intensity in adolescents with chronic fatigue syndrome (CFS). We then correlated the expression of these microRNAs with the levels of inflammatory markers and pain-related comorbidities in adolescents with CSF and healthy controls (HCs).<\/p>\n

METHODS:<\/p>\n

A total of 150 adolescents, aged 12-18 years, participated in this study between April 2016 and April 2017. The participants were classified into two groups: adolescents with CFS (n=100) and HCs (n=50). RT-PCR was used to evaluate the expression of miR-558, miR-146a, miR-150, miR-124, and miR-143. Immunoassay analysis was used to assess the levels of immune inflammatory markers IL-6, TNF-\u03b1, and COX-2.<\/p>\n

RESULTS:<\/p>\n

Adolescents with CFS showed significantly higher pain thresholds than comparable non-fatigued HCs. Also, enjoy of life and relation to others as the life domains, showed lower pain interference in CFS patients. Differential expression of miR-558, miR-146a, miR-150, miR-124, and miR-143 was significantly down regulated and notably interfered with pain intensity and frequency in patients with CFS. Also, the expression of these miRNAs was significantly correlated with that of IL-6, TNF-\u03b1, and COX-2, which have been shown to mediate pain intensity in patients with CFS.<\/p>\n

Girls with CSF showed significantly decreased expression levels of these miRNAs compared with the levels of boys with CSF. Girls with CSF also showed increased expression of inflammatory pain-related markers IL-6, TNF-\u03b1, and COX-2, compared with the levels of boys with CSF<\/p>\n

CONCLUSIONS:<\/p>\n

The intensity and consequences of pain were influenced by differential expression of miR-558, miR-146a, miR-150, miR-124, and miR-143, which was directly, associated with higher expression of immune inflammatory related genes TNF\u03b1, IL-6, and COX-2 in adolescences with CFS. Further studies of larger patient cohorts will help clarify the role of miRNAs in the pathogenesis of CFS.<\/p>\n

Read full paper<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

MicroRNAs as biomarkers of pain intensity in patients with chronic fatigue syndrome, by Hadeel A Al\u2010Rawaf, Ahmad H Alghadir, Sami A Gabr in Pain Pract. 2019 Jul 8. doi: 10.1111\/papr.12817. [Epub ahead of print] Research abstract: BACKGROUND: Numerous experimental models … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[625,5199,257,250,5197,18,5198,5201,1183,5200,427],"class_list":["post-21767","post","type-post","status-publish","format-standard","hentry","category-news","tag-adolescents","tag-ahmad-h-alghadir","tag-children","tag-chronic-pain","tag-dr-sami-a-gabr","tag-gene-expression","tag-hadeel-a-alrawaf","tag-immune-inflammatory-genes","tag-micrornas","tag-mirnas","tag-teenagers"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-5F5","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/21767","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=21767"}],"version-history":[{"count":1,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/21767\/revisions"}],"predecessor-version":[{"id":21768,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/21767\/revisions\/21768"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=21767"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=21767"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=21767"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}