{"id":22844,"date":"2019-11-05T09:24:26","date_gmt":"2019-11-05T09:24:26","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=22844"},"modified":"2019-11-05T09:24:26","modified_gmt":"2019-11-05T09:24:26","slug":"investigations-of-b-cell-phenotype-metabolic-function-in-patients-with-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/investigations-of-b-cell-phenotype-metabolic-function-in-patients-with-me-cfs\/","title":{"rendered":"Investigations of B cell phenotype & metabolic function in patients with ME\/CFS"},"content":{"rendered":"

Investigations of B cell phenotype and metabolic function in patients with Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome<\/a>, by Fane Kojo Fosu Mensah. PhD thesis University College London, Division of Medicine, Sep 2019<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a heterogeneous condition characterized by multiple systemic symptoms including fatigue, postexertional malaise and cognitive impairment, lasting for at least 6 months.<\/p>\n

Immune system dysfunction triggered by infection or other insult is generally assumed to be a major causal factor that contributes to changes in energy metabolism leading to the pathophysiology of ME\/CFS.<\/p>\n

B cells<\/a> became of interest after reported clinical improvement following B cell depletion-therapy with rituximab (anti-CD20). A possible but undefined role for B cells was, therefore, proposed.<\/p>\n

The initial aim of this thesis was to explore subtle alterations in B cell s\"Blausen.com<\/a>ubsets in ME\/CFS patients which could be used as a diagnostic and prognostic marker for the disease. Further, the dynamic nature of B cells was utilised as a model to observe changes in energy demand and for performing comprehensive metabolomic profiling of activated and maturating B cells under culture conditions.<\/p>\n

Results for HC and ME\/CFS patients could, therefore, be compared under similar conditions of stress. Flow-cytometric analysis of CD19+B cells revealed increased frequencies and expression of the heat-stable antigen CD24 in ME\/CFS patients, as well as an increased memory B cell subset (CD21+CD38-). Retention of CD24 was linked to unresponsiveness to proliferative and pro-apoptotic signals and phosphorylation of AMPK (pAMPK). PAMPK was found to be largely confined to IgD+IgM+ memory B cells.<\/p>\n

Metabolic analysis of cell culture supernatant using 1H-NMR spectroscopy revealed significant correlations between CD24+B cell frequencies and the usage of glucose and the production of lactate.<\/p>\n

Novel findings described in this thesis, therefore, established a link between CD24 positivity of B cells and energy metabolism. Immunophenotype and metabolite profiles of cultured B cells from HC and ME\/CFS patients were also revealed to respond with different dynamics to interventions, thereby providing a potential platform for more focused research and diagnosis.<\/p>\n","protected":false},"excerpt":{"rendered":"

Investigations of B cell phenotype and metabolic function in patients with Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome, by Fane Kojo Fosu Mensah. PhD thesis University College London, Division of Medicine, Sep 2019   Research abstract: Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[5357,5356,41,3601],"class_list":["post-22844","post","type-post","status-publish","format-standard","hentry","category-news","tag-b-cell-depletion","tag-dr-fane-kf-mensah","tag-energy-metabolism","tag-white-blood-cells"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-5Ws","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/22844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=22844"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/22844\/revisions"}],"predecessor-version":[{"id":22941,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/22844\/revisions\/22941"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=22844"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=22844"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=22844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}