{"id":23252,"date":"2019-12-09T23:47:50","date_gmt":"2019-12-09T23:47:50","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=23252"},"modified":"2019-12-09T23:47:50","modified_gmt":"2019-12-09T23:47:50","slug":"identification-of-actin-network-proteins-talin-1-filamin-a-in-circulating-extracellular-vesicles-as-blood-biomarkers-for-human-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/identification-of-actin-network-proteins-talin-1-filamin-a-in-circulating-extracellular-vesicles-as-blood-biomarkers-for-human-me-cfs\/","title":{"rendered":"Identification of actin network proteins, talin-1 &#038; filamin-A, in circulating extracellular vesicles as blood biomarkers for human ME\/CFS"},"content":{"rendered":"<h3><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0889159119307627\" target=\"_blank\" rel=\"noopener noreferrer\">Identification of actin network proteins, talin-1 and filamin-A, in circulating extracellular vesicles as blood biomarkers for human myalgic encephalomyelitis\/ chronic fatigue syndrome<\/a>, by Akiko Eguchi, Sanae Fukuda, Hirohiko Kuratsune, Junzo Nojima, Yasuhito Nakatomi, Yasuyoshi Watanabe, Ariel E Feldstein, <span style=\"text-decoration: underline;\">in<\/span> <em>Brain, Behavior, and Immunity\u00a0<\/em>November 2019 [\u00a0https:\/\/doi.org\/10.1016\/j.bbi.2019.11.015]<\/h3>\n<p>&nbsp;<\/p>\n<h3><strong>Highlights<\/strong><\/h3>\n<ul>\n<li>Circulating EV number was increased in ME\/CFS patients correlating to CRP and BAP.<\/li>\n<li>AUROC for circulating EVs was 0.802 allowing correct diagnosis in 90\u201394% of ME\/CFS.<\/li>\n<li>Proteins in actin skeletal regulation and EB virus infection were identified in ME\/CFS patients.<\/li>\n<li>Talin-1, filamin-A and 14-3-3 proteins were the most abundant proteins representing highly specific ME\/CFS.<\/li>\n<\/ul>\n<h3><strong>Research abstract:<\/strong><\/h3>\n<p>Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a serious, debilitating disorder with a wide spectrum of symptoms, including pain, depression, and neurocognitive deterioration. Over 17 million people around the world have ME\/CFS, predominantly women with peak onset at 30\u201350\u00a0years.<\/p>\n<p>Given the wide spectrum of symptoms and unclear etiology, specific biomarkers for diagnosis and stratification of ME\/CFS are lacking. Here we show that actin network proteins in circulating extracellular vesicles (EVs) offer specific non-invasive biomarkers for ME\/CFS.<\/p>\n<p><a href=\"https:\/\/wames.org.uk\/cms-english\/c-reactive-protein-response-in-patients-with-post-treatment-lyme-disease-symptoms-versus-those-with-me-cfs\/c-reative-protein\/\" rel=\"attachment wp-att-16897\"><img data-recalc-dims=\"1\" decoding=\"async\" class=\"alignright wp-image-16897 size-medium lazyload\" data-src=\"https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2018\/06\/C-reative-protein.jpg?resize=300%2C225&#038;ssl=1\" alt=\"\" width=\"300\" height=\"225\" data-srcset=\"https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2018\/06\/C-reative-protein.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2018\/06\/C-reative-protein.jpg?resize=150%2C113&amp;ssl=1 150w, https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2018\/06\/C-reative-protein.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2018\/06\/C-reative-protein.jpg?w=800&amp;ssl=1 800w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/225;\" \/><\/a>We found that circulating EVs were significantly increased in ME\/CFS patients correlating to C-reactive protein, as well as biological antioxidant potential. Area under the receiver operating characteristic curve for circulating EVs was 0.80, allowing correct diagnosis in 90\u201394% of ME\/CFS cases.<\/p>\n<p>From two independent proteomic analyses using circulating EVs from ME\/CFS, healthy controls, idiopathic chronic fatigue, and depression, proteins identified from ME\/CFS patients are involved in focal adhesion, actin skeletal regulation, PI3K-Akt signaling pathway, and Epstein-Barr virus infection. In particular, talin-1, filamin-A, and 14-3-3 family proteins were the most abundant proteins, representing highly specific ME\/CFS biomarkers.<\/p>\n<p>Our results identified circulating EV number and EV-specific proteins as novel biomarkers for diagnosing ME\/CFS, providing important information on the pathogenic mechanisms of ME\/CFS.<\/p>\n<p><a href=\"https:\/\/sci-hub.tw\/https:\/\/doi.org\/10.1016\/j.bbi.2019.11.015\" target=\"_blank\" rel=\"noopener noreferrer\">Read full paper<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Identification of actin network proteins, talin-1 and filamin-A, in circulating extracellular vesicles as blood biomarkers for human myalgic encephalomyelitis\/ chronic fatigue syndrome, by Akiko Eguchi, Sanae Fukuda, Hirohiko Kuratsune, Junzo Nojima, Yasuhito Nakatomi, Yasuyoshi Watanabe, Ariel E Feldstein, in Brain, &hellip; <a href=\"https:\/\/wames.org.uk\/cms-english\/identification-of-actin-network-proteins-talin-1-filamin-a-in-circulating-extracellular-vesicles-as-blood-biomarkers-for-human-me-cfs\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[5436,5443,5438,5437,731,221,224,5441,5440,5442,5439,759],"class_list":["post-23252","post","type-post","status-publish","format-standard","hentry","category-news","tag-actin-network-proteins","tag-akiko-eguchi","tag-ariel-e-feldstein","tag-circulating-evs","tag-crp","tag-ebv","tag-epstein-barr-virus","tag-hirohiko-kuratsune","tag-junzo-nojima","tag-sanae-fukuda","tag-yasuhito-nakatomi","tag-yasuyoshi-watanabe"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-632","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23252","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=23252"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23252\/revisions"}],"predecessor-version":[{"id":23386,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23252\/revisions\/23386"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=23252"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=23252"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=23252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}