{"id":23715,"date":"2019-12-28T22:23:08","date_gmt":"2019-12-28T22:23:08","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=23715"},"modified":"2020-01-27T22:27:34","modified_gmt":"2020-01-27T22:27:34","slug":"an-investigation-into-the-modulation-of-t-cell-phenotypes-by-amitriptyline-nortriptyline","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/an-investigation-into-the-modulation-of-t-cell-phenotypes-by-amitriptyline-nortriptyline\/","title":{"rendered":"An investigation into the modulation of T cell phenotypes by amitriptyline & nortriptyline"},"content":{"rendered":"

An investigation into the modulation of T cell phenotypes by amitriptyline and nortriptyline<\/a>, by JonathanRoyds, Melissa J Conroy, Margaret R Dunne, Connail McCrory, Joanne Lysaght in<\/span> European Neuropsychopharmacology, <\/em>Dec 2019[doi.org\/10.1016\/j.euroneuro.2019.12.106]<\/p>\n

 <\/p>\n

Research abstract:<\/strong>
\nAmitriptyline<\/a> is prescribed for treating the symptoms of neuroinflammatory disorders including neuropathic pain and fibromyalgia. As amitriptyline has evidence of modulating the neuroimmune interface; the effects of amitriptyline treatment on T-cell phenotype and function were examined in vitro<\/a>. Peripheral blood mononuclear cells<\/a> (PBMCs) were isolated and treated with amitriptyline, nortriptyline<\/a> and a combination of both drugs. Toxicity for T-cells was assessed by Annexin V\/Propidium Iodide staining. Activation status and cytokine expression by T-cells post treatment was assessed by flow cytometry.<\/p>\n

The levels of secreted cytokines, chemokines and neurotrophins were measured by ELISA in the supernatants. There was no significant increase in T-cell death following 24 or 48\u00a0h compared to controls. There were significantly lower frequencies of CD8+ T-cells after treatment with amitriptyline, nortriptyline and a combination of both compared to a Vehicle Control(VC)(p<0.001). The frequencies of naive CD8+CD45RA+ cells were significantly lower after amitriptyline, nortriptyline and a combination of both (p<0001).<\/p>\n

The frequencies of CD27+CD4+(p<0.05) and CD27+CD8+(p<0.01) T-cells were also significantly lower following combination drug treatment. Significantly lower frequencies of IFN-\u03b3-producing CD8+ T-cells were observed with all treatment combinations(p<0.05) and frequencies of IL-17-producing CD4+ and CD8+ T-cells were significantly lower following amitriptyline treatment (p<0.05).<\/p>\n

Frequencies of Natural Killer T-cells<\/a> were significantly higher following treatment with nortriptyline (p<0.05). Significantly higher levels of IL-16 (p<0.001) and lower levels of TNF-\u03b2 (p<0.05) were observed in supernatants. This data indicates that both amitriptyline and nortriptyline modulate the phenotype and function of T-cells and this may have clinical relevance in the pathologies of its off-label applications.<\/p>\n

Read full paper<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

An investigation into the modulation of T cell phenotypes by amitriptyline and nortriptyline, by JonathanRoyds, Melissa J Conroy, Margaret R Dunne, Connail McCrory, Joanne Lysaght in European Neuropsychopharmacology, Dec 2019[doi.org\/10.1016\/j.euroneuro.2019.12.106]   Research abstract: Amitriptyline is prescribed for treating the symptoms … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1950,5548,5547,5551,2330,5549,5550,722,705,615,5546,2892,2893],"class_list":["post-23715","post","type-post","status-publish","format-standard","hentry","category-news","tag-amitriptyline","tag-connail-mccrory","tag-joanne-lysaght","tag-jonathanroyds","tag-killer-t-cells","tag-margaret-r-dunne","tag-melissa-j-conroy","tag-natural-killer-cells","tag-neuroinflammation","tag-neuropathic-pain","tag-nortriptyline","tag-pbmc","tag-peripheral-blood-mononuclear-cells"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-6av","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23715","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=23715"}],"version-history":[{"count":6,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23715\/revisions"}],"predecessor-version":[{"id":24083,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/23715\/revisions\/24083"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=23715"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=23715"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=23715"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}