{"id":24317,"date":"2020-02-17T21:49:45","date_gmt":"2020-02-17T21:49:45","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=24317"},"modified":"2020-02-17T21:52:38","modified_gmt":"2020-02-17T21:52:38","slug":"assessing-diagnostic-value-of-micrornas-from-peripheral-blood-mononuclear-cells-extracellular-vesicles-in-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/assessing-diagnostic-value-of-micrornas-from-peripheral-blood-mononuclear-cells-extracellular-vesicles-in-me-cfs\/","title":{"rendered":"Assessing diagnostic value of microRNAs from peripheral blood mononuclear cells & extracellular vesicles in ME\/CFS"},"content":{"rendered":"

Assessing diagnostic value of microRNAs from peripheral blood mononuclear cells and extracellular vesicles in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome<\/a> by Eloy Almenar-P\u00e9rez, Leonor Sarr\u00eda, Lubov Nathanson & Elisa Oltra in<\/span> Scientific Reports<\/em> vol 10, no: 2064 (2020)<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS) is a debilitating multisystemic disease of unknown etiology, affecting thousands of individuals worldwide. Its diagnosis still relies on ruling out medical problems leading to unexplained fatigue due to a complete lack of disease-specific biomarkers.<\/p>\n

\"\"<\/a>Our group and others have explored the potential value of microRNA profiles<\/a> (miRNomes) as diagnostic tools for this disease. However, heterogeneity of participants, low numbers, the variety of samples assayed, and other pre-analytical variables, have hampered the identification of\u00a0 disease-associated miRNomes.<\/p>\n

In this study, our team has evaluated, for the first time, ME\/CFS miRNomes in peripheral blood mononuclear cells<\/a> (PBMCs) and extracellular vesicles<\/a> (EVs) from severely ill patients recruited at the monographic UK ME biobank to assess, using standard operating procedures (SOPs), blood fractions with optimal diagnostic power for a rapid translation of a miR-based diagnostic method into the clinic.<\/p>\n

Our results show that routine creatine kinase<\/a> (CK) blood values, plasma EVs physical characteristics (including counts, size and zeta-potential), and a limited number of differentially expressed PBMC and EV miRNAs appear significantly associated with severe ME\/CFS (p\u2009<\u20090.05).<\/p>\n

Gene enrichment analysis points to epigenetic<\/a> and neuroimmune<\/a> dysregulated pathways, in agreement with previous reports. Population validation by a cost-effective approach limited to these few potentially discriminating variables is granted.<\/p>\n

 <\/p>\n

Comment: <\/strong>MEA Summary Review: Assessing diagnostic value of microRNAs from PMBC\u2019s and EV\u2019s in ME\/CFS<\/a>\u00a0 11 February 2020<\/p>\n","protected":false},"excerpt":{"rendered":"

Assessing diagnostic value of microRNAs from peripheral blood mononuclear cells and extracellular vesicles in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome by Eloy Almenar-P\u00e9rez, Leonor Sarr\u00eda, Lubov Nathanson & Elisa Oltra in Scientific Reports vol 10, no: 2064 (2020)   Research abstract: Myalgic … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[4971,4977,4385,4281,4543,4546,5617,5616,2892,2893,3377,36],"class_list":["post-24317","post","type-post","status-publish","format-standard","hentry","category-news","tag-creatine-kinase","tag-eloy-almenar-perez","tag-evs","tag-extracellular-vesicles","tag-leonor-sarria","tag-lubov-nathanson","tag-microrna-profiles","tag-mirnomes","tag-pbmc","tag-peripheral-blood-mononuclear-cells","tag-prof-elisa-oltra","tag-severe-me"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-6kd","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/24317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=24317"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/24317\/revisions"}],"predecessor-version":[{"id":24471,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/24317\/revisions\/24471"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=24317"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=24317"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=24317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}