{"id":27745,"date":"2020-10-07T08:24:13","date_gmt":"2020-10-07T07:24:13","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=27745"},"modified":"2020-10-07T08:24:13","modified_gmt":"2020-10-07T07:24:13","slug":"a-swath-ms-analysis-of-me-cfs-peripheral-blood-mononuclear-cell-proteomes-reveals-mitochondrial-dysfunction","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/a-swath-ms-analysis-of-me-cfs-peripheral-blood-mononuclear-cell-proteomes-reveals-mitochondrial-dysfunction\/","title":{"rendered":"A SWATH-MS analysis of ME\/CFS peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction"},"content":{"rendered":"

A SWATH-MS analysis of ME\/CFS peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction<\/a>, by Eiren Sweetman, Torsten Kleffmann, Christina Edgar, Michel de Lange, Rosamund Vallings &\u00a0Warren Tate in<\/span> Journal of Translational Medicine<\/em> vol 18, no: 365 (2020) [doi.org\/10.1186\/s12967-020-02533-3]<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Background:<\/strong>
\nMyalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS) is a serious and complex physical illness that affects all body systems with a multiplicity of symptoms, but key hallmarks of the disease are pervasive fatigue and \u2018post-exertional malaise\u2019, exacerbation after physical and\/or mental activity of the intrinsic fatigue and other symptoms that can be highly debilitating and last from days to months.<\/p>\n

Although the disease can vary widely between individuals, common symptoms also include pain, cognitive deficits, sleep dysfunction, as well as immune, neurological and autonomic symptoms. Typically, it is a very isolating illness socially, carrying a stigma because of the lack of understanding of the cause and pathophysiology.<\/p>\n

Methods:<\/strong>
\nTo gain insight into the pathophysiology of ME\/CFS, we examined the proteomes<\/a> of peripheral blood mononuclear cells<\/a> (PBMCs) by SWATH-MS analysis<\/a> in a small well-characterised group of patients and matched controls. A principal component analysis (PCA) was used to stratify groups based on protein abundance patterns, which clearly segregated the majority of the ME\/CFS patients (9\/11) from the controls. This majority subgroup of ME\/CFS patients was then further compared to the control group.<\/p>\n

Results:<\/strong>
\n\"\"A total of 60 proteins in the ME\/CFS patients were differentially expressed (P\u2009<\u20090.01, Log10\u00a0(Fold Change)\u2009>\u20090.2 and\u2009<\u2009\u22120.2). Comparison of the PCA selected subgroup of ME\/CFS patients (9\/11) with controls increased the number of proteins differentially expressed to 99. Of particular relevance to the core symptoms of fatigue and post-exertional malaise experienced in ME\/CFS, a proportion of the identified proteins in the ME\/CFS groups were involved in mitochondrial function, oxidative phosphorylation, electron transport chain complexes, and redox regulation. A significant number were also involved in previously implicated disturbances in ME\/CFS, such as the immune inflammatory response, DNA methylation, apoptosis and proteasome activation.<\/p>\n

Conclusions:<\/strong>
\nThe results from this study support a model of deficient ATP production in ME\/CFS, compensated for by upregulation of immediate pathways upstream of Complex V that would suggest an elevation of oxidative stress. This study and others have found evidence of a distinct pathology in ME\/CFS that holds promise for developing diagnostic biomarkers.<\/p>\n","protected":false},"excerpt":{"rendered":"

A SWATH-MS analysis of ME\/CFS peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction, by Eiren Sweetman, Torsten Kleffmann, Christina Edgar, Michel de Lange, Rosamund Vallings &\u00a0Warren Tate in Journal of Translational Medicine vol 18, no: 365 (2020) [doi.org\/10.1186\/s12967-020-02533-3]   Research … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[3283,175,4849,1320,4851,6238,168,2892,2893,4117,6239],"class_list":["post-27745","post","type-post","status-publish","format-standard","hentry","category-news","tag-adenosine-triphosphate","tag-atp-profile-test","tag-christina-edgar","tag-dr-rosamund-vallings","tag-eiren-sweetman","tag-michel-de-lange","tag-mitochondrial-dysfunction","tag-pbmc","tag-peripheral-blood-mononuclear-cells","tag-prof-warren-tate","tag-torsten-kleffmann"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-7dv","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/27745","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=27745"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/27745\/revisions"}],"predecessor-version":[{"id":28224,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/27745\/revisions\/28224"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=27745"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=27745"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=27745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}