{"id":28795,"date":"2020-12-11T03:14:55","date_gmt":"2020-12-11T03:14:55","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=28795"},"modified":"2020-12-11T03:14:55","modified_gmt":"2020-12-11T03:14:55","slug":"impact-of-long-term-cryopreservation-on-blood-immune-cell-markers-in-me-cfs-implications-for-biomarker-discovery","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/impact-of-long-term-cryopreservation-on-blood-immune-cell-markers-in-me-cfs-implications-for-biomarker-discovery\/","title":{"rendered":"Impact of long-term cryopreservation on blood immune cell markers in ME\/CFS: implications for biomarker discovery"},"content":{"rendered":"

Impact of long-term cryopreservation on blood immune cell markers in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: implications for biomarker discovery<\/a>, by Elisabet G\u00f3mez-Mora,\u00a0 Jorge Carrillo,\u00a0 V\u00edctor Urrea,\u00a0 Josepa Rigau,\u00a0 Jos\u00e9 Alegre,\u00a0 Cecilia Cabrera,\u00a0 Elisa Oltra,\u00a0 Jes\u00fas Castro-Marrero and\u00a0 Juli\u00e0 Blanco in<\/span> Front. Immunol.<\/em>, 17 November 2020 [doi.org\/10.3389\/fimmu.2020.582330]<\/h3>\n

 <\/p>\n

\"\"Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS) is a complex neuroimmune disorder<\/a> characterized by numerous symptoms of unknown etiology<\/a>. The ME\/CFS immune markers reported so far have failed to generate a clinical consensus, perhaps partly due to the limitations of biospecimen biobank<\/a>.<\/p>\n

To address this issue, we performed a comparative analysis of the impact of long-term biobanking on previously identified immune markers and also explored additional potential immune markers linked to infection in ME\/CFS. A correlation analysis of marker cryostability<\/a> across immune cell subsets based on flow cytometry immunophenotyping of fresh blood and frozen PBMC<\/a> samples collected from individuals with ME\/CFS (n = 18) and matched healthy controls (n\u00a0= 18) was performed.<\/p>\n

The functionality of biobanked samples was assessed on the basis of cytokine production assay after stimulation of frozen PBMCs. T cell markers defining Treg subsets and the expression of surface glycoprotein CD56 in T cells and the frequency of the effector CD8 T cells, together with CD57 expression in NK cells, appeared unaltered by biobanking. By contrast, NK cell markers CD25 and CD69 were notably increased, and NKp46 expression markedly reduced, by long-term cryopreservation and thawing. Further exploration of Treg and NK cell subsets failed to identify significant differences between ME\/CFS patients and healthy controls in terms of biobanked PBMCs.<\/p>\n

Our findings show that some of the previously identified immune markers in T and NK cell subsets become unstable after cell biobanking, thus limiting their use in further immunophenotyping<\/a> studies for ME\/CFS. These data are potentially relevant for future multisite intervention studies and cooperative projects for biomarker discovery using ME\/CFS biobanked samples. Further studies are needed to develop novel tools for the assessment of biomarker stability in cryopreserved immune cells from people with ME\/CFS.<\/p>\n","protected":false},"excerpt":{"rendered":"

Impact of long-term cryopreservation on blood immune cell markers in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: implications for biomarker discovery, by Elisabet G\u00f3mez-Mora,\u00a0 Jorge Carrillo,\u00a0 V\u00edctor Urrea,\u00a0 Josepa Rigau,\u00a0 Jos\u00e9 Alegre,\u00a0 Cecilia Cabrera,\u00a0 Elisa Oltra,\u00a0 Jes\u00fas Castro-Marrero and\u00a0 Juli\u00e0 Blanco in Front. … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1364,726,6391,6395,6394,6396,4284,4289,6392,6390,691,2892,2893,3377,6393],"class_list":["post-28795","post","type-post","status-publish","format-standard","hentry","category-news","tag-biobank","tag-biomarkers","tag-cecilia-cabrera","tag-cryopreservation","tag-elisabet-gomez-mora","tag-freezing","tag-jesus-castro-marrero","tag-jose-alegre","tag-josepa-rigau","tag-julia-blanco","tag-nk-cells","tag-pbmc","tag-peripheral-blood-mononuclear-cells","tag-prof-elisa-oltra","tag-victor-urrea"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-7ur","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/28795","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=28795"}],"version-history":[{"count":3,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/28795\/revisions"}],"predecessor-version":[{"id":29044,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/28795\/revisions\/29044"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=28795"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=28795"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=28795"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}