{"id":29134,"date":"2021-01-25T08:53:05","date_gmt":"2021-01-25T08:53:05","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=29134"},"modified":"2021-01-25T08:53:05","modified_gmt":"2021-01-25T08:53:05","slug":"multi-omics-examination-of-q-fever-fatigue-syndrome-identifies-similarities-with-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/multi-omics-examination-of-q-fever-fatigue-syndrome-identifies-similarities-with-cfs\/","title":{"rendered":"Multi-omics examination of Q fever fatigue syndrome identifies similarities with CFS"},"content":{"rendered":"

Multi-omics examination of Q fever fatigue syndrome identifies similarities with chronic fatigue syndrome<\/a>, by Ruud PH Raijmakers, Megan E Roerink, Anne FM Jansen, Stephan P Keijmel, Ranko Gacesa, Yang Li, Leo AB Joosten, Jos WM van der Meer, Mihai G Netea, Chantal P Bleeker-Rovers & Cheng-Jian Xu in<\/span> Journal of Translational Medicine<\/span><\/em> vol 18, Article: 448 (2020)<\/h3>\n

 <\/p>\n

Research abstract:\u00a0<\/strong><\/h3>\n

Background:<\/strong>
\nQ fever fatigue syndrome<\/a> (QFS) is characterised by a state of prolonged fatigue that is seen in 20% of acute Q fever infections and has major health-related consequences. The molecular mechanisms underlying QFS are largely unclear. In order to better understand its pathogenesis, we applied a multi-omics approach<\/a> to study the patterns of the gut microbiome, blood metabolome, and inflammatory proteome of QFS patients, and compared these with those of chronic fatigue syndrome (CFS) patients and healthy controls (HC).<\/p>\n

Methods<\/strong>
\nThe study population consisted of 31 QFS patients, 50 CFS patients, and 72 HC. All subjects were matched for age, gender, and general geographical region (South-East part of the Netherlands). The gut microbiome composition was assessed by Metagenomic sequencing using the Illumina HiSeq platform. A total of 92 circulating inflammatory markers were measured using Proximity Extension Essay and 1607 metabolic features were assessed with a high-throughput non-targeted metabolomics approach.<\/p>\n

\"\"

Protein MMP-1<\/p><\/div>\n

Results<\/strong>
\nInflammatory markers<\/strong>, including 4E-BP1 (P\u2009=\u20099.60\u201316\u00a0and 1.41\u20137) and MMP-1 (P\u2009=\u20097.09\u20139\u00a0and 3.51\u20139), are significantly more expressed in both QFS and CFS patients compared to HC. Blood metabolite profiles<\/strong> show significant differences when comparing QFS (319 metabolites) and CFS (441 metabolites) patients to HC, and are significantly enriched in pathways like sphingolipid (P\u2009=\u20090.0256 and 0.0033) metabolism.<\/p>\n

When comparing QFS to CFS patients, almost no significant differences in metabolome<\/strong> were found. Comparison of microbiome taxonomy of QFS and CFS patients with that of HC, shows both in- and decreases in abundancies in Bacteroidetes (with emphasis on Bacteroides and Alistiples spp.), and Firmicutes and Actinobacteria (with emphasis n Ruminococcus and Bifidobacterium spp.). When we compare QFS patients to CFS patients, there is a striking resemblance and hardly any significant differences in microbiome taxonomy<\/strong> are found.<\/p>\n

Conclusions<\/strong>
\nWe show that QFS and CFS patients are similar across three different omics layers and 4E-BP1<\/a> and MMP-1<\/a> have the potential to distinguish QFS and CFS patients from HC.<\/p>\n","protected":false},"excerpt":{"rendered":"

Multi-omics examination of Q fever fatigue syndrome identifies similarities with chronic fatigue syndrome, by Ruud PH Raijmakers, Megan E Roerink, Anne FM Jansen, Stephan P Keijmel, Ranko Gacesa, Yang Li, Leo AB Joosten, Jos WM van der Meer, Mihai G … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[6464,5067,6460,6461,5065,3299,5066,6465,6467,6466,1999,6463,5061,5063,6462],"class_list":["post-29134","post","type-post","status-publish","format-standard","hentry","category-news","tag-anne-fm-jansen","tag-chantal-p-bleeker-roversen","tag-cheng-jian-xu","tag-jos-wm-van-der-meer","tag-leo-ab-joosten","tag-megan-e-roerink","tag-mihai-g-netea","tag-multi-omics","tag-protein-4e-bp1","tag-protein-mmp-1","tag-q-fever-fatigue-syndrome","tag-ranko-gacesa","tag-ruud-ph-raijmakers","tag-stephan-p-keijmel","tag-yang-li"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-7zU","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/29134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=29134"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/29134\/revisions"}],"predecessor-version":[{"id":29518,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/29134\/revisions\/29518"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=29134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=29134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=29134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}