{"id":30011,"date":"2021-03-04T21:33:24","date_gmt":"2021-03-04T21:33:24","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=30011"},"modified":"2021-03-08T12:26:02","modified_gmt":"2021-03-08T12:26:02","slug":"recursive-ensemble-feature-selection-provides-a-robust-mrna-expression-signature-for-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/recursive-ensemble-feature-selection-provides-a-robust-mrna-expression-signature-for-me-cfs\/","title":{"rendered":"Recursive ensemble feature selection provides a robust mRNA expression signature for ME\/CFS"},"content":{"rendered":"

Recursive ensemble feature selection provides a robust mRNA expression signature for myalgic encephalomyelitis\/chronic fatigue syndrome<\/a>, by Paula I Metselaar, Lucero Mendoza-Maldonado, Andrew Yung Fong Li Yim, Ilias Abarkan, Peter Henneman, Anje A Te Velde, Alexander Sch\u00f6nhuth, Jos A Bosch, Aletta D Kraneveld, Alejandro Lopez-Rincon in<\/span>\u00a0Sci Rep<\/em>. 2021 Feb 25;11(1):4541 [doi: 10.1038\/s41598-021-83660-9]<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a chronic disorder characterized by disabling fatigue. Several studies have sought to identify diagnostic biomarkers, with varying results.<\/p>\n

\"\"

Representation of a DNA molecule that is methylated. The two white spheres represent methyl groups. They are bound to two cytosine nucleotide molecules that make up the DNA sequence.<\/p><\/div>\n

Here, we innovate this process by combining both mRNA<\/a> expression and DNA methylation<\/a> data. We performed recursive ensemble feature selection<\/a> (REFS) on publicly available mRNA expression data in peripheral blood mononuclear cells<\/a> (PBMCs) of 93 ME\/CFS patients and\u00a025 healthy controls, and found a signature of 23 genes capable of distinguishing cases and controls<\/strong>. REFS highly outperformed other methods, with an AUC of 0.92.<\/p>\n

We validated the results on a different platform (AUC of 0.95) and in DNA methylation\u00a0data obtained from four public studies on ME\/CFS (99 patients and 50 controls), identifying 48 gene-associated CpGs that predicted disease status as well (AUC of 0.97). Finally, ten of the 23 genes could be interpreted in the context of the derailed immune system of ME\/CFS<\/strong>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Recursive ensemble feature selection provides a robust mRNA expression signature for myalgic encephalomyelitis\/chronic fatigue syndrome, by Paula I Metselaar, Lucero Mendoza-Maldonado, Andrew Yung Fong Li Yim, Ilias Abarkan, Peter Henneman, Anje A Te Velde, Alexander Sch\u00f6nhuth, Jos A Bosch, Aletta … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[6630,6631,6633,6639,6634,2007,6636,6632,6638,6643,1166,6637,2892,2893,6635,6645,6644],"class_list":["post-30011","post","type-post","status-publish","format-standard","hentry","category-news","tag-alejandro-lopez-rincon","tag-aletta-d-kraneveld","tag-alexander-schonhuth","tag-andrew-yung-fong-li-yim","tag-anje-a-te-velde","tag-dna-methylation","tag-ilias-abarkan","tag-jos-a-bosch","tag-lucero-mendoza-maldonado","tag-messenger-rna","tag-mrna","tag-paula-i-metselaar","tag-pbmc","tag-peripheral-blood-mononuclear-cells","tag-peter-henneman","tag-recursive-ensemble-feature-selection","tag-refs"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-7O3","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30011","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=30011"}],"version-history":[{"count":3,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30011\/revisions"}],"predecessor-version":[{"id":30061,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30011\/revisions\/30061"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=30011"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=30011"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=30011"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}