{"id":30364,"date":"2021-07-09T08:29:28","date_gmt":"2021-07-09T07:29:28","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=30364"},"modified":"2022-01-19T07:44:47","modified_gmt":"2022-01-19T07:44:47","slug":"skewing-of-the-b-cell-receptor-repertoire-in-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/skewing-of-the-b-cell-receptor-repertoire-in-me-cfs\/","title":{"rendered":"Biomarker research: Skewing of the B cell receptor repertoire in ME\/CFS"},"content":{"rendered":"<h3><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0889159121001537\" target=\"_blank\" rel=\"noopener\">Skewing of the B cell receptor repertoire in myalgic encephalomyelitis\/ chronic fatigue syndrome<\/a>, by\u00a0Wakiro Sato, Hirohiko Ono, Takaji Matsutani, Masakazu Nakamura, Isu Shin, Keiko Amano, Ryuji Suzuki, Takashi Yamamura <span style=\"text-decoration: underline;\">in<\/span> <em>Brain, Behavior, and Immunity<\/em>, Vol 95, July 2021, Pages 245-255<\/h3>\n<p>&nbsp;<\/p>\n<p><strong>Highlights<\/strong><\/p>\n<ul>\n<li>ME\/CFS is characterized by skewed B cell receptor gene usage.<\/li>\n<li>Upregulation of specific IGHV genes correlated to infection-related episodes at onset.<\/li>\n<li>Plasmablasts of ME\/CFS patients upregulated interferon response genes.<\/li>\n<li>B cell receptor repertoire analysis can provide a useful diagnostic marker in ME\/CFS.<\/li>\n<\/ul>\n<p><strong>Research abstract:<\/strong><\/p>\n<p>Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a debilitating condition characterized by fatigue and post-exertional malaise, accompanied by various signs of neurological and autonomic dysfunction. ME\/CFS is often triggered by an infectious episode and associated with an aberrant immune system.<\/p>\n<div id=\"attachment_31841\" style=\"width: 288px\" class=\"wp-caption alignright\"><img data-recalc-dims=\"1\" decoding=\"async\" aria-describedby=\"caption-attachment-31841\" class=\"wp-image-31841 size-medium lazyload\" data-src=\"https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2021\/07\/B-cell-receptor.jpg?resize=278%2C300&#038;ssl=1\" alt=\"By CNX OpenStax - http:\/\/cnx.org\/contents\/GFy_h8cu@10.53:rZudN6XP@2\/Introduction, CC BY 4.0, https:\/\/commons.wikimedia.org\/w\/index.php?curid=49935883\" width=\"278\" height=\"300\" data-srcset=\"https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2021\/07\/B-cell-receptor.jpg?resize=278%2C300&amp;ssl=1 278w, https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2021\/07\/B-cell-receptor.jpg?resize=139%2C150&amp;ssl=1 139w, https:\/\/i0.wp.com\/wames.org.uk\/cms-english\/wp-content\/uploads\/2021\/07\/B-cell-receptor.jpg?w=544&amp;ssl=1 544w\" data-sizes=\"(max-width: 278px) 100vw, 278px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 278px; --smush-placeholder-aspect-ratio: 278\/300;\" \/><p id=\"caption-attachment-31841\" class=\"wp-caption-text\">Structure of the B cell receptor<\/p><\/div>\n<p>Here we report that ME\/CFS is a disorder characterized by skewed <a href=\"https:\/\/en.wikipedia.org\/wiki\/B-cell_receptor\" target=\"_blank\" rel=\"noopener\">B cell receptor<\/a> gene usage. By applying a next-generation sequencing to determine the clone-based IGHV\/IGHD\/IGHJ repertoires, we revealed a biased usage of several IGHV genes in peripheral blood B cells from ME\/CFS patients. Results of receiver operating characteristic (ROC) analysis further indicated a possibility of distinguishing patients from healthy controls, based on the skewed B cell repertoire.<\/p>\n<p>Meanwhile, B cell clones using IGHV3-30 and IGHV3-30-3 genes were more frequent in patients with an obvious infection-related episode at onset, and correlated to expression levels of interferon response genes in <a href=\"https:\/\/en.wikipedia.org\/wiki\/Plasma_cell\" target=\"_blank\" rel=\"noopener\">plasmablasts<\/a>.<\/p>\n<p>Collectively, these results imply that B cell responses in ME\/CFS are directed against an infectious agents or priming antigens induced before disease onset.<\/p>\n<h3><strong>From the Discussion:<\/strong><\/h3>\n<p>In conclusion, we demonstrated that specific BCR IGH usage is selectively upregulated in ME\/CFS patients compared with HCs. Among the upregulated genes, IGHV3-30\/3-30-3 are associated with an infection-related episode before disease onset, with relatively short disease duration and with the expression of type 1 IFN response genes in PB. BCR repertoire analysis could be developed as a powerful tool to help diagnose ME\/CFS. It may also be used to predict the efficacy of B cell-targeted therapy, which is effective in a subgroup of patients.<\/p>\n<p><strong>NCNP<\/strong>: <a href=\"https:\/\/www.ncnp.go.jp\/topics\/2021\/20210520eng.html\" target=\"_blank\" rel=\"noopener\">Discovery of Immune Biomarkers for Myalgic Encephalomyelitis \/ Chronic Fatigue Syndrome (ME\/CFS) based on B cell receptor repertoire analysis<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Skewing of the B cell receptor repertoire in myalgic encephalomyelitis\/ chronic fatigue syndrome, by\u00a0Wakiro Sato, Hirohiko Ono, Takaji Matsutani, Masakazu Nakamura, Isu Shin, Keiko Amano, Ryuji Suzuki, Takashi Yamamura in Brain, Behavior, and Immunity, Vol 95, July 2021, Pages 245-255 &hellip; <a href=\"https:\/\/wames.org.uk\/cms-english\/skewing-of-the-b-cell-receptor-repertoire-in-me-cfs\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[6899,4170,6901,6898,6895,6043,6896,6894,6900,6897,6893,6049],"class_list":["post-30364","post","type-post","status-publish","format-standard","hentry","category-news","tag-b-cell-receptor","tag-biomarker","tag-gene","tag-hirohiko-ono","tag-isu-shin","tag-keiko-amano","tag-masakazu-nakamura","tag-ryuji-suzuki","tag-skewed-b-cell-receptor-gene-use","tag-takaji-matsutani","tag-takashi-yamamura","tag-wakiro-sato"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-7TK","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30364","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=30364"}],"version-history":[{"count":7,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30364\/revisions"}],"predecessor-version":[{"id":33955,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/30364\/revisions\/33955"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=30364"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=30364"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=30364"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}