{"id":31749,"date":"2021-07-08T07:17:32","date_gmt":"2021-07-08T06:17:32","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=31749"},"modified":"2022-02-18T07:23:44","modified_gmt":"2022-02-18T07:23:44","slug":"bioenergetic-proteomic-profiling-of-immune-cells-in-me-cfs-patients","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/bioenergetic-proteomic-profiling-of-immune-cells-in-me-cfs-patients\/","title":{"rendered":"Bioenergetic & proteomic profiling of immune cells in ME\/CFS patients"},"content":{"rendered":"

Bioenergetic and proteomic profiling of immune cells in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome Patients: an exploratory study<\/a>, by Paula Fernandez-Guerra, Ana C Gonzalez-Ebsen, Susanne E Boonen, Julie Courraud, Niels Gregersen, Jesper Mehlsen, Johan Palmfeldt, Rikke K J Olsen, Louise Schouborg Brinth in<\/span> Biomolecules<\/em> 2021 Jun 29;11(7):961 [doi: 10.3390\/biom11070961]<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a heterogeneous, debilitating, and complex disease. Along with disabling fatigue, ME\/CFS presents an array of other core symptoms, including autonomic nervous system (ANS) dysfunction, sustained inflammation, altered energy metabolism, and mitochondrial dysfunction.<\/p>\n

\"\"Here, we evaluated patients’ symptomatology and the mitochondrial metabolic parameters in peripheral blood mononuclear cells<\/a> (PBMCs) and plasma<\/a> from a clinically well-characterised cohort of six ME\/CFS patients compared to age- and gender-matched controls.<\/p>\n

We performed a comprehensive cellular assessment using bioenergetics (extracellular flux analysis<\/a>) and protein profiles (quantitative mass spectrometry-based proteomics<\/a>) together with self-reported symptom measures of fatigue, ANS dysfunction, and overall physical and mental well-being.<\/p>\n

This ME\/CFS cohort presented with severe fatigue, which correlated with the severity of ANS dysfunction and overall physical well-being. PBMCs from ME\/CFS patients showed significantly lower mitochondrial coupling efficiency<\/strong>. They exhibited proteome<\/a> alterations, including altered mitochondrial<\/a> metabolism, centred on pyruvate dehydrogenase and coenzyme A metabolism, leading to a decreased capacity to provide adequate intracellular ATP levels.<\/p>\n

Overall, these results indicate that PBMCs from ME\/CFS patients have a decreased ability to fulfill their cellular energy demands.<\/p>\n

From study Discussion (4):<\/strong><\/h3>\n

…in this study, we aimed to recruit a homogenous group of ME\/CFS patients and applied strategies for personalised medicine by performing many tests in a few individuals to shed light on molecular mechanisms of disease in these individuals.<\/p>\n

Despite the small sample size, our data indicate a dysregulated mitochondrial metabolism<\/strong> centred on PDH and CoA metabolism, which supports findings from other and larger ME\/CFS cohorts [29].<\/p>\n

Interestingly, between 35 and 40% of ME\/CFS patients experience significant improvement in their health when treated with dichloroacetate<\/a>, a well-known activator of PDH [74,75]. These open-label studies need further follow-up; however, together with the present study and the previous metabolomics and proteomic studies of independent ME\/CFS cohorts, they support dysregulated mitochondrial metabolism as an important feature of ME\/CFS pathology.<\/p>\n

To some degree, abnormalities in bioenergetics parameters of blood cells have shown to correlate with the severity of ME\/CFS symptoms [31,76]. Similarly, coupling efficiency correlated with symptom severity and disease duration in our cohort of ME\/CFS patients.<\/p>\n","protected":false},"excerpt":{"rendered":"

Bioenergetic and proteomic profiling of immune cells in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome Patients: an exploratory study, by Paula Fernandez-Guerra, Ana C Gonzalez-Ebsen, Susanne E Boonen, Julie Courraud, Niels Gregersen, Jesper Mehlsen, Johan Palmfeldt, Rikke K J Olsen, Louise Schouborg Brinth … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[6889,6891,6885,6884,6887,6882,168,6886,6890,2892,2893,6892,6883,6888],"class_list":["post-31749","post","type-post","status-publish","format-standard","hentry","category-news","tag-ana-c-gonzalez-ebsen","tag-extracellular-flux-analysis","tag-jesper-mehlsen","tag-johan-palmfeldt","tag-julie-courraud","tag-louise-schouborg-brinth","tag-mitochondrial-dysfunction","tag-niels-gregersen","tag-paula-fernandez-guerra","tag-pbmc","tag-peripheral-blood-mononuclear-cells","tag-quantitative-mass-spectrometry-based-proteomics","tag-rikke-k-j-olsen","tag-susanne-e-boonen"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-8g5","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/31749","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=31749"}],"version-history":[{"count":4,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/31749\/revisions"}],"predecessor-version":[{"id":34183,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/31749\/revisions\/34183"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=31749"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=31749"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=31749"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}