{"id":32131,"date":"2021-11-15T10:09:26","date_gmt":"2021-11-15T10:09:26","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=32131"},"modified":"2021-12-09T20:24:52","modified_gmt":"2021-12-09T20:24:52","slug":"research-the-characterization-of-il-2-stimulation-treatment-of-trpm3-in-me-cfs","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/research-the-characterization-of-il-2-stimulation-treatment-of-trpm3-in-me-cfs\/","title":{"rendered":"Research: The characterization of IL-2 stimulation & treatment of TRPM3… in ME\/CFS"},"content":{"rendered":"

Characterization of IL-2 stimulation and TRPM7 pharmacomodulation in NK Cell cytotoxicity and channel Co-Localization with PIP2 in Myalgic Encephalomyelitis\/ Chronic Fatigue Syndrome patients<\/a>, by Stanley Du Preez , Natalie Eaton-Fitch, Helene Cabanas, Donald Staines and Sonya Marshall-Gradisnik in<\/span> Int. J. Environ. Res. Public Health<\/em> 2021, 18(22), 11879; [doi.org\/10.3390\/ijerph182211879] 12 Nov 2021\u00a0 (This article belongs to the Special Issue\u00a0Chronic Fatigue Syndrome: Medical, Nursing and Public Health Management)<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a complex multisystemic disorder responsible for significant disability. Although a unifying etiology for ME\/CFS is uncertain, impaired natural killer (NK) cell cytotoxicity<\/a> represents a consistent and measurable feature of this disorder.<\/p>\n

Research utilizing patient-derived NK cells has implicated dysregulated calcium (Ca2+) signaling<\/a>, dysfunction of the phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent cation channel, transient receptor potential melastatin (TRPM) 3, as well as altered surface expression patterns of TRPM3 and TRPM2 in the pathophysiology of ME\/CFS.<\/p>\n

TRPM7<\/a> is a related channel that is modulated by PIP2 and participates in Ca2+ signaling. Though TRPM7 is expressed on NK cells, the role of TRPM7 with IL-2 and intracellular signaling mechanisms in the NK cells of ME\/CFS patients is unknown.<\/p>\n

This study examined the effect of IL-2<\/a> stimulation and TRPM7 pharmacomodulation on NK cell cytotoxicity using flow cytometric assays as well as co-localization of TRPM7 with PIP2 and cortical actin using confocal microscopy in 17 ME\/CFS patients and 17 age- and sex-matched healthy controls.<\/p>\n

The outcomes of this investigation are preliminary and indicate that crosstalk between IL-2 and TRMP7 exists. A larger sample size to confirm these findings and characterization of TRPM7 in ME\/CFS using other experimental modalities are warranted.<\/p>\n","protected":false},"excerpt":{"rendered":"

Characterization of IL-2 stimulation and TRPM7 pharmacomodulation in NK Cell cytotoxicity and channel Co-Localization with PIP2 in Myalgic Encephalomyelitis\/ Chronic Fatigue Syndrome patients, by Stanley Du Preez , Natalie Eaton-Fitch, Helene Cabanas, Donald Staines and Sonya Marshall-Gradisnik in Int. J. … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1249,4308,7147,5376,7145,691,7146,2676,5375,7090,7148],"class_list":["post-32131","post","type-post","status-publish","format-standard","hentry","category-news","tag-dr-sonya-marshall-gradisnik","tag-helene-cabanas","tag-il-2","tag-natalie-eaton-fitch","tag-national-killer-cell","tag-nk-cells","tag-pip2","tag-prof-donald-staines","tag-stanley-du-preez","tag-transient-receptor-potential-melastatin-7","tag-trpm7"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-8mf","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/32131","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=32131"}],"version-history":[{"count":7,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/32131\/revisions"}],"predecessor-version":[{"id":33732,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/32131\/revisions\/33732"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=32131"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=32131"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=32131"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}