{"id":34296,"date":"2022-02-23T22:07:24","date_gmt":"2022-02-23T22:07:24","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=34296"},"modified":"2022-02-23T22:07:24","modified_gmt":"2022-02-23T22:07:24","slug":"treatment-research-restoration-using-naltrexone-in-natural-killer-cells-of-me-cfs-patients","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/treatment-research-restoration-using-naltrexone-in-natural-killer-cells-of-me-cfs-patients\/","title":{"rendered":"Treatment research: …restoration using Naltrexone in natural killer cells of ME\/CFS patients"},"content":{"rendered":"

Impaired TRPM3-dependent calcium influx and restoration using Naltrexone in natural killer cells of myalgic encephalomyelitis\/chronic fatigue syndrome patients<\/a>, by Natalie Eaton-Fitch, Stanley Du Preez, H\u00e9l\u00e8ne Cabanas, Katsuhiko Muraki, Donald Staines & Sonya Marshall-Gradisnik in<\/span> Journal of Translational Medicine<\/em> volume 20, Article number: 94 (2022)<\/h3>\n

 <\/p>\n

Research abstract:<\/strong><\/h3>\n

Background:<\/strong>
\nMyalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a serious disorder of unknown aetiology. While the pathomechanism of ME\/CFS remains elusive, reduced natural killer (NK) cell cytotoxic function is a consistent immunological feature. NK cell effector functions rely on long-term sustained calcium (Ca2+) influx.<\/p>\n

\"\"In recent years evidence of transient receptor potential melastatin 3 (TRPM3)<\/a> dysfunction supports the hypothesis that ME\/CFS is potentially an ion channel disorder<\/a>. Specifically, reports of single nucleotide polymorphisms, low surface expression and impaired function of TRPM3 have been reported in NK cells of ME\/CFS patients.<\/p>\n

It has been reported that mu (\u00b5)-opioid receptor (\u00b5OR) agonists, known collectively as opioids<\/a>, inhibit TRPM3. Naltrexone hydrochloride (NTX)<\/a>, a \u00b5OR antagonist, negates the inhibitory action of \u00b5OR on TRPM3 function. Importantly, it has recently been reported that NTX restores impaired TRPM3 function in NK cells of ME\/CFS patients.<\/p>\n

Methods<\/strong>
\nLive cell immunofluorescent imaging was used to measure TRPM3-dependent Ca2+\u00a0influx in NK cells isolated from n\u2009=\u200910 ME\/CFS patients and n\u2009=\u200910 age- and sex-matched healthy controls (HC) following modulation with TRPM3-agonist, pregnenolone sulfate (PregS) and TRPM3-antaognist, ononetin. The effect of overnight (24\u00a0h) NTX in vitro treatment on TRPM3-dependent Ca2+\u00a0influx was determined.<\/p>\n

Results<\/strong>
\nThe amplitude (p\u2009<\u20090.0001) and half-time of Ca2+\u00a0response (p\u2009<\u20090.0001) was significantly reduced at baseline in NK cells of ME\/CFS patients compared with HC. Overnight treatment of NK cells with NTX significantly improved TRPM3-dependent Ca2+\u00a0influx in ME\/CFS patients. Specifically, there was no significance between HC and ME\/CFS patients for half-time response, and the amplitude of Ca2+\u00a0influx was significantly increased in ME\/CFS patients (p\u2009<\u20090.0001).<\/p>\n

Conclusion<\/strong>
\nTRPM3-dependent Ca2+\u00a0influx was restored in ME\/CFS patients following overnight treatment of isolated NK cells with NTX in vitro. <\/strong><\/p>\n

Collectively, these findings validate that TRPM3 loss of function results in altered Ca2+\u00a0influx supporting the growing evidence that ME\/CFS is a TRP ion channel disorder and that NTX<\/a> provides a potential therapeutic intervention for ME\/CFS.<\/strong><\/p>\n

Research features<\/strong>: Understanding the intricacies of ion channels in ME\/chronic fatigue syndrome<\/a> – The background to the work of Griffith University’s research into ion channel dysfunction in ME\/CFS and their search for treatments.<\/p>\n","protected":false},"excerpt":{"rendered":"

Impaired TRPM3-dependent calcium influx and restoration using Naltrexone in natural killer cells of myalgic encephalomyelitis\/chronic fatigue syndrome patients, by Natalie Eaton-Fitch, Stanley Du Preez, H\u00e9l\u00e8ne Cabanas, Katsuhiko Muraki, Donald Staines & Sonya Marshall-Gradisnik in Journal of Translational Medicine volume 20, … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1249,4308,4597,5351,5376,722,691,2676,5375,5350],"class_list":["post-34296","post","type-post","status-publish","format-standard","hentry","category-news","tag-dr-sonya-marshall-gradisnik","tag-helene-cabanas","tag-katsuhiko-muraki","tag-naltrexone-hydrochloride","tag-natalie-eaton-fitch","tag-natural-killer-cells","tag-nk-cells","tag-prof-donald-staines","tag-stanley-du-preez","tag-trpm3-ion-channel-function"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-8Va","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/34296","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=34296"}],"version-history":[{"count":1,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/34296\/revisions"}],"predecessor-version":[{"id":34298,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/34296\/revisions\/34298"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=34296"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=34296"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=34296"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}