{"id":36405,"date":"2022-08-20T09:58:40","date_gmt":"2022-08-20T08:58:40","guid":{"rendered":"https:\/\/wames.org.uk\/cms-english\/?p=36405"},"modified":"2022-09-12T10:44:54","modified_gmt":"2022-09-12T09:44:54","slug":"research-impaired-trpm3-ion-channel-function-found-in-me-cfs-long-covid","status":"publish","type":"post","link":"https:\/\/wames.org.uk\/cms-english\/research-impaired-trpm3-ion-channel-function-found-in-me-cfs-long-covid\/","title":{"rendered":"Research: Impaired TRPM3 ion channel function found in ME\/CFS & Long COVID"},"content":{"rendered":"

TRPM3 dysfunction in post COVID-19 condition and ME\/CFS patients<\/strong><\/h2>\n

 <\/p>\n

Research from the NCNED<\/a> in Australia, using their Electrophysiology ‘Patch Clamp’ technique, concludes:<\/strong><\/p>\n

The results of this investigation suggest that post COVID-19 condition patients may have impaired TRPM3<\/a> ion channel function and provide further evidence regarding the similarities between post COVID-19 condition and ME\/CFS.<\/p>\n

Impaired TRPM3 channel activity in post COVID-19 condition patients suggest impaired ion mobilisation which may consequently impede cell function resulting in chronic post-infectious symptoms.<\/p>\n

Further investigation into TRPM3 function may elucidate the pathomechanism, provide a diagnostic and therapeutic target for post COVID-19 condition patients and commonalities with ME\/CFS patients.<\/p>\n

\"\"<\/p>\n

 <\/p>\n

Research abstract:\u00a0<\/strong><\/h3>\n

Background<\/strong>:<\/p>\n

Myalgic encephalomyelitis\/chronic fatigue syndrome\u00a0(ME\/CFS) is a severe multisystemic condition associated with post-infectious onset, impaired natural killer (NK) cell<\/a> cytotoxicity and impaired ion channel function<\/a>, namely Transient Receptor Potential Melastatin 3<\/a> (TRPM3). Long-term effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has resulted in neurocognitive, immunological, gastrointestinal, and cardiovascular manifestations recently recognised as post coronavirus disease 2019\u00a0(COVID-19) condition. The symptomatology of ME\/CFS overlaps significantly with post COVID-19; therefore, this research aimed to investigate TRPM3 ion channel function in post COVID-19 condition patients.<\/p>\n

Methods: <\/strong><\/p>\n

Whole-cell patch-clamp technique was used to measure TRPM3 ion channel activity in isolated NK cells of N\u2009=\u20095 ME\/CFS patients, N =\u20095 post COVID-19 patients, and N\u2009=\u20095 healthy controls (HC). The TRPM3 agonist, pregnenolone sulfate (PregS) was used to activate TRPM3 function, while ononetin was used as a TRPM3 antagonist.<\/p>\n

Results:<\/strong><\/p>\n

As reported in previous research, PregS-induced TRPM3 currents were significantly reduced in ME\/CFS patients compared with HC (p\u2009=\u20090.0048). PregS-induced TRPM3 amplitude was significantly reduced in post COVID-19 condition compared with HC (p\u2009=\u20090.0039).<\/p>\n

Importantly, no significant difference was reported in ME\/CFS patients compared with post COVID-19 condition as PregS-induced TRPM3 currents of post COVID-19 condition patients were similar of ME\/CFS patients currents (p\u2009>\u20090.9999). Isolated NK cells from post COVID-19 condition and ME\/CFS patients were resistant to ononetin and differed significantly with HC (p\u2009<\u20090.0001).<\/p>\n

Conclusion: <\/strong><\/p>\n

The results of this investigation suggest that post\u00a0COVID-19 condition patients may have impaired TRPM3 ion channel function and provide further evidence regarding the similarities between post COVID-19 condition and ME\/CFS. Impaired TRPM3 channel activity in post COVID-19 condition patients suggest impaired ion mobilisation which may consequently impede cell function resulting in chronic post-infectious symptoms. Further investigation into TRPM3 function may elucidate the pathomechanism, provide a diagnostic and therapeutic target for post COVID-19 condition patients and commonalities with ME\/CFS patients.<\/p>\n

 <\/p>\n

Read full paper:<\/strong><\/h3>\n

Transient receptor potential melastatin 3 dysfunction in post COVID-19 condition and myalgic encephalomyelitis\/chronic fatigue syndrome patients<\/a>, by Etianne Martini Sasso, Katsuhiko Muraki, Natalie Eaton-Fitch, Peter Smith, Olivia Ly Lesslar, Gary Deed & Sonya Marshall-Gradisnik in\u00a0<\/span>Molecular Medicine<\/em> volume 28, Article number: 98 (2022)<\/p>\n

\"\"Comment from NCNED<\/strong>: World first chronic fatigue syndrome findings could fast track response to Long COVID<\/a><\/p>\n

\u201cOur researchers have pioneered a specialised technique known as electrophysiology or \u2018patch-clamp\u2019 in immune cells. This technique previously led the team to report on the pathology of ME\/CFS and to examine specific ion channels in cells.”<\/p>\n","protected":false},"excerpt":{"rendered":"

TRPM3 dysfunction in post COVID-19 condition and ME\/CFS patients   Research from the NCNED in Australia, using their Electrophysiology ‘Patch Clamp’ technique, concludes: The results of this investigation suggest that post COVID-19 condition patients may have impaired TRPM3 ion channel … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[1249,7338,7339,4934,4597,6196,5376,7337,6909,6183,5350],"class_list":["post-36405","post","type-post","status-publish","format-standard","hentry","category-news","tag-dr-sonya-marshall-gradisnik","tag-etianne-martini-sasso","tag-gary-deed","tag-immune-cell-dysregulation","tag-katsuhiko-muraki","tag-long-covid","tag-natalie-eaton-fitch","tag-olivia-ly-lesslar","tag-patch-clamp-electrophysiology","tag-peter-smith","tag-trpm3-ion-channel-function"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5qkYK-9tb","_links":{"self":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/36405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/comments?post=36405"}],"version-history":[{"count":6,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/36405\/revisions"}],"predecessor-version":[{"id":36678,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/posts\/36405\/revisions\/36678"}],"wp:attachment":[{"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/media?parent=36405"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/categories?post=36405"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wames.org.uk\/cms-english\/wp-json\/wp\/v2\/tags?post=36405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}